Avtor/Urednik		Vodopivec, M; Berovič, M; Jančar, J; Podgornik, A; Štrancar, A
Naslov		Application of convective interaction media disks with immobilised glucose oxidase for on-line glucose measurements
Tip		članek
Vir		Anal Chim Acta
Vol. in št.		Letnik 407
Leto izdaje		2000
Obseg		str. 105-10
Jezik		eng
Abstrakt		A new polymeric macroporous material, Convective Interaction Media (CIM) was appliedas a support for glucose oxidase (GOD) immobilisation. CIM epoxy disks with the immobilised enzyme were integrated as an enzyme reactor in a flow injection analysis (FIA) system and applied to on-line monitoring of glucose during cultivation of Saccharomyces cerevisiae and citric acid production by Aspergillus niger. The developed CIM GOD disk-FIA system exhibited good signal reproducibility and satisfactory long-term stability with a liner response in the range 10-200 mg l-1. The CIM disk applied as an enzyme reactor proved to be a successful replacement for conventionally used packed-bed columns and as such it was well suited for on-line monitoring of bioprocesses.
Deskriptorji		SACCHAROMYCES CEREVISIAE ASPERGILLUS NIGER GLUCOSE GLUCOSE OXIDASE ENZYMES, IMMOBILIZED CULTURE MEDIA FERMENTATION