Avtor/Urednik		Žerovnik, Eva; Jerala, Roman; Kroon-Žitko, Louisa; Turk, Vito; Pain, Roger H
Naslov		Denaturation of stefin B by GuHCl, pH and heat: evidence for molten globule intermediates
Tip		članek
Vir		Biol Chem Hoppe Seyler
Vol. in št.		Letnik 373, št. 7
Leto izdaje		1992
Obseg		str. 453-8
Jezik		eng
Abstrakt		GuHCl, pH and thermal denaturation of the recombinant stefin B was followed by circular dichroism (CD) and size-exclusion chromatography (SEC). CD at 277 nm was taken as an indicator of integral tertiary structure and CD at 222 nm as an indicator of the secondary structure. Compactness was expressed by the volumes of elution on a SEC (Superose 12) column. Data on equilibrium denaturation were recalculated to the fractions of the native state (fN). The results have shown that equilibrium intermediates of the molten globule type exist under conditions of low pH, high temperature or medium GuHCl concentrations, namely A, T and G. Recent findings on structure and energetics of molten globule intermediates are reviewed.
Deskriptorji		CYSTATINS GUANIDINES CIRCULAR DICHROISM HEAT HYDROGEN-ION CONCENTRATION PROTEIN CONFORMATION PROTEIN DENATURATION RECOMBINANT PROTEINS SPECTROPHOTOMETRY, INFRARED SPECTROPHOTOMETRY, ULTRAVIOLET