Avtor/Urednik		Žerovnik, Eva; Jerala, Roman; Kroon-Žitko, Louisa; Pain, Roger H; Turk, Vito
Naslov		Intermediates in denaturation of a small globular protein, recombinant human stefin B
Tip		članek
Vir		J Biol Chem
Vol. in št.		Letnik 267, št. 13
Leto izdaje		1992
Obseg		str. 9041-6
Jezik		eng
Abstrakt		Guanidinium HCl (GdmHCl), pH, and heat denaturation of the recombinant human stefin B, a low molecular weight protein inhibitor of cysteine proteinases, has been followed by circular dichroism. From the noncoincidence of the transitions in the near and far UV, the existence of stable intermediate states possessing few persistent tertiary interactions but most of the native-like secondary structure, was inferred. These intermediate states exist at equilibrium under various conditions, namely, state G at 1.7 M GdmHCl (pH 8, 25 degrees C), state A at pH 4 (0.6 M GdmHCl, 25 degrees C) and state T above 68 degrees C. By size exclusion chromatography, their apparent compactness was determined. The intermediate states A, T, and G were compact and are therefore classified as "molten globule" states.
Deskriptorji		CYSTATINS CHROMATOGRAPHY, GEL CIRCULAR DICHROISM GUANIDINES HEAT HYDROGEN-ION CONCENTRATION PROTEIN DENATURATION RECOMBINANT PROTEINS SPECTROPHOTOMETRY, ULTRAVIOLET