Avtor/Urednik     Mulec, Janez; Starčič, Marjanca; Žgur-Bertok, Darja
Naslov     F-like plasmid sequences in enteric bacteria of diverse origin, with implication of horizontal transfer and plasmid host range
Tip     članek
Vir     Curr Microbiol
Vol. in št.     Letnik 44
Leto izdaje     2002
Obseg     str. 231-5
Jezik     eng
Abstrakt     Seventy-eight bacterial isolates from human, animal, and plant hosts, representing eight species of the family Enterobacteriaceae, were screened for F-like plasmid sequences. Of the'examined human Escherichia coli strains, 28% harbored one or two of the three F-like, RepFI replication regions, while 35% of the examined animal and all phytopathogenic strains harbored RepFIA-specific sequences. Comparative analysis of Salmonella, Shigella, Erwinia, and E. coli plasmid RepFI sequences showed 100% or very high homology, indicating frequent and recent interspecies gene transfer. The high incidence of RepFIA sequences in enteric bacterial species, including Klebsiella and Erwinia, showed that F-like plasmids are successful in avoiding natural barriers to establishment of horizontally transferred DNA and that in the natural environment conjugal transfer is efficient in diverse ecological niches.
Deskriptorji     ENTEROBACTERIACEAE
PLASMIDS
F FACTOR
ESCHERICHIA COLI
GENE TRANSFER
POLYMERASE CHAIN REACTION
REPLICON
SHIGELLA
SALMONELLA
KLEBSIELLA
ERWINIA
SEROTYPING