Avtor/Urednik		Kendall, Edward; Gong, Zhao; Tuchek, John; Obenaus, Andre
Naslov		Physical and chemical caharacteristics of neurological injury in an avian model of primary generalized epilepsy
Tip		članek
Vir		Image Anal Stereol
Vol. in št.		Letnik 21, št. 2
Leto izdaje		2002
Obseg		str. 77-86
Jezik		eng
Abstrakt		Purpose This study was undertaken to determine if the epileptic chicken phenotype exhibited unique physical and chemical neurological changes. Methods Quantitative 1H-MRI/MRS measurements were performed on adult control (Rr) and epileptic (rr) chickens. The metrics included nuclear relaxation rate, diffusion rate and concentration of metabolites associated with neuronal cells. Comparisons were performed using Students t or Mann-Whitney tests according to the data distribution. Results Proton density measurements detected no significant difference in density among the cerebral hemispheres and optic lobes within the control and epi birds, nor between bird phenotypes. Thus, within experimental error it appears that brain tissue density is similar in adult control and epi birds. Volume calculation from images spanning the entire brain confirmed that a consistent feature of the epi genotype was megalencephaly. Nuclear relaxation (T2 ) values obtained for the control bird were within the normal variant range for neuronal tissue. However, T2's of the epi birds were significantly higher than that of the controls ((158 ms versus 123 ms). Similarly, in the diffusion data there was no apparent hemispheric bias. The optic lobes of the control animals exhibited an apparent diffusion coefficient significantly lower than that found for the cerebrum (Table 2). Interestingly, this differential was not apparent in the epi birds. The control birds across all regions of interest exhibited an ADC significantly less than that of the epi birds. Quantitative 'Hspectroscopy using tissue extracts collected immediately after sacrifice revealed phenotypic differences in lactate, glutamate, creatine and NAA but not GABA. A reduction in NAA was detected in the cerebrum of epi birds. (Abstract truncated at 2000 characters).
Deskriptorji		EPILEPSY, GENERALIZED MAGNETIC RESONANCE IMAGING NUCLEAR MAGNETIC RESONANCE BRAIN CHEMISTRY DISEASE MODELS, ANIMAL CHICKENS PHENOTYPE WATER DIFFUSION CEREBELLUM OPTIC LOBE