Avtor/Urednik		Leštan, Domen; Černilec, Maja; Perdih, Anton
Naslov		Determination of ligninase activity in P. chrysosporium pellets with diffuse reflectance spectrophotometry
Tip		članek
Vir		Appl Microbiol Biotechnol
Vol. in št.		Letnik 38
Leto izdaje		1993
Obseg		str. 570-3
Jezik		eng
Abstrakt		Diffuse reflectance spectrophotometry was applied for measuring ligninase activity in pellets of Phanerochaete chrysosporium. Enhanced ligninase activity in pellets and in growth medium were detected in cultures supplemented with oleic acid emulsified with Tween 80, Tween 80, hydrophilic (alcoholic) residue of Tween 80 hydrolysate, Tween 20, and (150E)C18:1. In cultures with low extracellular ligninase activity, low activity in pellets was also observed. Our results indicate that the stimulatory effect of the tested surfactants cannot be contributed solely to promotion of ligninases through the cell membrane.
Deskriptorji		BASIDIOMYCETES LIGNIN FERMENTATION SPECTROPHOTOMETRY