Avtor/Urednik		Levičar, Nataša; Strojnik, Tadej; Kos, Janko; Dewey, Ricardo A; Pilkington, Geoffrey J; Lah, Tamara T
Naslov		Lysosomal enzymes, cathepsins in brain tumour invasion
Tip		članek
Vir		J Neurooncol
Vol. in št.		Letnik 58, št. 1
Leto izdaje		2002
Obseg		str. 21-32
Jezik		eng
Abstrakt		The expression patterns of different classes of peptidases in central nervous system (CNS) tumours have been most extensively studied in astrocytomas and meningiomas. Although the two types of tumours are very different in mtist respects, both may invade locally into normal brain. This process of invasion includes increased synthesis and secretion of lysosomal proteolytic enzymes - cathepsins. Aspartic endopeptidase cathepsin (Cat) D levels were found to be elevated in high-grade astrocytoma and partial lhhibition of glioblastoma cell invasion by anti-Cat D antibody suggests that the enzyme activity is involved in the invasion process. Several studies on cysteine endopeptidase (CP) Cat B in gliomas agreed that transcript abundance, protein level and activity of Cat B increased in high-grade astrocytoma cultures compared with low-grade astrocytoma cultures and normal brain. Moreover, in glioma biopsies Cat B levels correlated with evidence of clinical invasion and it has been demonstrated that Cat B both in tumour cells and in endothelial cells can serve as a new biological marker for prognosis in glioblastoma patients. A high level of Cat B protein was also a diagnostic marker for invasive types of meningioma, distinguishing between histomorphologically benign, but invasive meningiomas and noninvasive, so-called clear-benign meningiomas. Cat L was also significantly increased in high-grade astrocytoma compared with low-grade astrocytoma and normal brain. Specific Cat L antibodies and antisense Cat L RNA transfection significantly lowered glioblastoma cell invasion. In meningioma, Cat L was a less-significant marker of invasion than Cat B. (Abstract truncated at 2000 characters)
Deskriptorji		BRAIN NEOPLASMS NEOPLASM INVASIVENESS CATHEPSINS CYSTATINS CYSTEINE PROTEINASE INHIBITORS GLIOMA MENINGIOMA GLIOBLASTOMA ASTROCYTOMA PROGNOSIS