Avtor/Urednik		Minshall, Richard D.; Pavčnik, Dušan; Halushka, Perry V; Hermsmeyer, Kent
Naslov		Progesterone regulation of vascular thromboxane A2 receptors in rhesus monkeys
Tip		članek
Vir		Am J Physiol, Heart Circ Physiol
Vol. in št.		Letnik 281
Leto izdaje		2001
Obseg		str. H1498-507
Jezik		eng
Abstrakt		We hypothesized that progesterone regulates thromboxane A2 receptor (TxA2R) density in primate vascular muscle and that TxA2R density correlates with coronary reactivity in vivo and in vitro. Reactivity to serotonin + U-46619 was determined by angiography in surgically postmenopausal (ovariectomized (Ovx)) rhesus monkeys without progesterone replacement and after 2-wk progesterone treatment (1-2 ng/ml). In untreated Ovx animals, 100 micromol/1 serotonin + 1 micromol/1 U-46619 (syringe concentrations) provoked vasospasm-like constrictions in six of six monkeys; zero of six progesterone-treated monkeys developed vasospasms. Sustained Ca2+ responses in vascular muscle cells isolated from Ovx coronaries (208 +- 63% of basal 20 min after stimulation) treated with serotonin + U-46619 contrasted with transient Caz+ responses (143 +- 18% of basal and decreasing 5 min after stimulation) in progesterone treated monkeys. The maximum density of (1S-(11,2J(5Z),31(lE,3R*),4I)-7-(3'-(3-hydroxy-4-(4'-(125l)iodophenoxy)1-butenyl)-7-oxabicyclo(2.2.1)heptan-2-yl)-5-heptenoic acid ((125I)-BOP) binding was greater (P < 0.01) in carotid arteries and aortic membranes from Ovx (109 +- 11 fmol/mg) compared with progesterone-treated (43 +- 15 fmol/mg) monkeys. TxA2R immunolabeling revealed greater coronary TxAaR labeling in Ovx compared with progesterone-treated monkeys. The results suggest that progesterone can decrease arterial TxA2R in Ovx monkeys.
Deskriptorji		PROGESTERONE RECEPTORS, THROMBOXANE OVARIECTOMY SEROTONIN ENDOTHELIUM, VASCULAR CORONARY VASOSPASM ENDOTHELIN-1 ARACHIDONIC ACID RADIOLIGAND ASSAY