Avtor/Urednik		Vlaisavljević, Veljko; Kovačič, Borut; Reljič, Milan; Gavrić-Lovrec, Vida; Čižek-Sajko, Mojca
Naslov		Is there any benefit from the culture of a single oocyte to a blastocyst-stage embryo in unstimulated cycles?
Tip		članek
Vir		Hum Reprod
Vol. in št.		Letnik 16, št. 11
Leto izdaje		2001
Obseg		str. 2379-83
Jezik		eng
Abstrakt		Background: The aim of the study was to test the influence of 2- and 5-day cultivation of a single oocyte on the pregnancy rate in a non-stimulated cycle. Methods: A retrospective chart review of 391 consecutive patients undergoing IVF and intracytoplasmic sperm injection in unstimulated cycles was performed. The embryos were kept in MediCult universal IVF medium for day 2 transfers and in BlastAssist System for day 5 transfers. Results: The oocyte recovery rate in the group for 2-day cultivation and in the group for 5-day cultivation was similar, being 79.4 (162/204) and 83.6% (154/187) respectively. The same is true of the fertilization rate (73.8 versus 77.7%). The blastulation rate was 52.8%. The embryo transfer rate per cycle was higher when day 2 embryos were transferred: 64.8% (105/162) compared with 35.7% (55/154) if blastocyst-stage embryos were transferred. The pregnancy rate per transferred embryo was higher when a blastocyst was transferred (40.0%) instead of a day 2 embryo (23.8%). CONCLUSION: The expected pregnancy rate calculated per embryo available on day 2 of cultivation was similar in both groups (23.8 versus 22.2 % ) and it was not affected by oocyte culture to the blastocyst stage.
Deskriptorji		FERTILIZATION IN VITRO EMBRYO TRANSFER BLASTOCYST OOCYTES SPERMATOZOA RETROSPECTIVE STUDIES CULTURE MEDIA TISSUE CULTURE