Avtor/Urednik		Knuplež, Uršula
Naslov		Iskanje različno izraženih genov v mišjih linijah (Mus musculus), ki se razlikujejo v kvantitativnem lokusu za debelost
Prevedeni naslov		Screening for differentially expressed genes in mouse lines differing by an obesity quantitative trait locus
Tip		monografija
Kraj izdaje		Ljubljana
Založnik		Medicinska fakulteta
Leto izdaje		2002
Obseg		str. 94
Jezik		slo
Abstrakt		Obesity is a complex trait determined by the action of several genetic and environmental factors. The genetic determinants are genes; involved in the control of energy balance, nutrient partitioning, lipid metabolism and behavior. The central nervous system influences energy balance and body weight. Hypothalamus is a critica.l region of the brain for regulation of homeostatic processes. The sensory inputs are relayed to regulatory sites in the hypothalamus where the information is integrated and a neuro-humoral response initiated. Various hormones, neuropeptides and neurotransmitters are involved in this regulatory loop. The genetic basis of obesity is mostly polygenic. The development of obesity is controlled by several genomic regions named quantitative trait loci (QTL). Rodent shains undergoing longterm divergent seleetion for obesity-related traits are useful models to characterize the complex genetic basis of obesity. Mice selected for high (F line) and low (L line) body fat content were used in our study. In a previous study, several obesity QTLs were identified in this mouse model. A genomic segment containing QTL that mapped to chromosome 15 was transferred by repeated backcrossing from the L line into the genetic background of the F line resulting in a congenic line (F1sL line). To examine which genes are differentially expressed in the hypothalami of the F and F1sL lines differential display analysis was applied. Additionally, northern analysis of adequate tissues was used to identify differentially expressed positional candidate genes, which map to likelihood interval containing the obesity QTL on chromosome 1 S. Knowledge of differentially expressed genes should help in the identification of a metabolic pathway perturbed in the F1sL line of mice. (Abstract truncated at 2000 characters).
Deskriptorji		OBESITY HYPOTHALAMUS POLYMORPHISM (GENETICS) GENE EXPRESSION RNA, MESSENGER POLYMERASE CHAIN REACTION POLYMORPHISM, SINGLE-STRANDED CONFORMATIONAL BASE SEQUENCE BLOTTING, NORTHERN BLOTTING, SOUTHERN MICE, OBESE