Avtor/Urednik		Juntes, P; Zabavnik-Piano, J; Čurin-Šerbec, V; Kovač, Z; Pogačnik, M
Naslov		Neuropathological lesions in the first case of bovine spongiform encephalopathy in Slovenia
Prevedeni naslov		Nevropatološke spremembe pri prvem primeru goveje spongiformne encefalopatije v Sloveniji
Tip		članek
Vir		Slov Vet Res
Vol. in št.		Letnik 39, št. 2
Leto izdaje		2002
Obseg		str. 105-14
Jezik		slo, eng
Abstrakt		The first Slovenian case of bovine spongiform encephalopathy (BSE) was diagnosed in November 2001. The diseased animal, a five-year-old cow of brown breed, was killed because of its general weakness and sent in a rendering plant to be destroyed. A sample of the cow's medulla oblongata was taken to be examined in a fast post-mortem test which is part of a systematic surveillance program for BSE in fallen stock over 24 months of age. The medulla oblongata sample tested positive during a Prionics Check Western test. This was later confirmed histopathologically, with the presence of spongiform lesions in the brain, and with a positive immunohistochemical reaction for prions using a set of monoclonal antibodies: C1/1:G9, K4B3:F9, V5B2 and E12/2:F6. The diagnosis was also confirmed in the reference laboratory for transmissible spongiform encephalopathies in Bern (Switzerland) with the second fast post-mortem test, histopathologically and immunohistochemically.
Izvleček		Novembra 2001 je bil v Sloveniji ugotovljen prvi primer goveje spongiformne encefalopatije (BSE). Bolezen se je pojavila pri pet let stari kravi sivorjave pasme, ki je bila ubita zaradi splošnega slabega stanja in poslana v neškodljivo uničenje. Vzorec za preiskavo na BSE je bil vzet ob rednem sistematičnem pregledu poginjenih goved, starejših od 24 mesecev, s hitrim postmortalnim testom. V testu Prionics Check Western je bila podaljšana hrbtenjača te živali močno pozitivna. Bolezen je bila potrjena z ugotovitvijo značilnih spongiformnih sprememb v možganih in pozitivno imunohistokemično reakcijo na prione z monoklonskimi protitelesi C1/1:G9, K4B3:F9, V5B2 in E12/2:F6. Diagnoza je bila potrjena v referenčnem laboratoriju za prenosljive spongiformne encefalopatije v Bernu (Švica) z drugim hitrim testom ter s patohistološko in imunohistokemično preiskavo.
Deskriptorji		ENCEPHALOPATHY, BOVINE SPONGIFORM IMMUNOHISTOCHEMISTRY AUTOPSY CATTLE