Avtor/Urednik		Cabadaj, R; Nagy, J; Popelka, P; Mate, D; Bugarsky, A
Naslov		The determination of salinomycin residues in the tissues of broiler chickens by using microbiological diffusion methods
Prevedeni naslov		Ugotavljanje ostankov salinomicina v tkivih brojlerskih prašičev z mikrobiološkimi difuzijskimi metodami
Tip		članek
Vir		Slov Vet Res
Vol. in št.		Letnik 39, št. 2
Leto izdaje		2002
Obseg		str. 137-43
Jezik		eng
Abstrakt		Two hundred three-day-old broiler chickens (ROSS hybrid) were used in the experiment. Salinomycin (Synvertas, Biotika Slovenska L'upča, The Slovak Republic) was orally administered to three groups of chickens in dosages of 600 mg/kg-1 of feed (60 mg salinomycin - group I); 900 mg/kg-1 of feed (90 mg salinomycin - group II); 1,800 mg/kg-1 of feed (180 mg salinomycin - group III). Sacox 120 (12 % salinomycin, Biotika Slovenska L'upča) was administered to the fourth group of chickens (500 mg/kg-1 feed; 60 mg of salinomycin). The birds were treated until they were 42 days old. Then 70 chickens were randomly selected and killed; 6 birds from each experimental group (I-IV) on the first day after the end of the treatment and two birds from each experimental group on the 2nd, 3rd, 4th, Sth, 6th and 7th days. Breast and thigh muscles, liver, gizzard and heart samples were analysed microbiologically, with Bacillus stearothermophillus var. calidolactis C 953 as a test micro-organism, and by the four-plate method. The four-plate method was more sensitive than the Bac. stearothermophillus microbiological method to the residues of salinomycin, which were detectable in the chicken tissues for between 4 and 6 days, depending on the dosage that they received.
Izvleček		V poskusu smo uporabili dvesto tri dni starih brojlerskih piščancev (križanci ross). Piščance smo krmili z dodatkom salinomicina (synvertas, Biotika Slovenska L'upča, Slovaška) v odmerkih 600 mg/kg krme (60 mg salinomicina - skupina I), 900 mg/kg krme (90 mg salinomicina - skupina II) in 1800 mg/kg krme (180 mg salinomicina - skupina III). Četrti skupini piščancev smo dodajali sacox 120 (12% salinomicina, Biotika Slovenskž L'upča) v odmerku 500 mg/kg krme, 60 mg salinomicina). Piščance smo tako tretirali do 42. dneva starosti. Nato smo naključno izbrali in žrtvovali 70 živali: dan po končanem eksperimentalnem krmljenju po 6 živali iz vsake skupine (I-IV), nato pa po dve živali iz vsake skupine 2., 3., 4., 5., 6. in 7. dan. Jemali smo vzorce prsnih in stegenskih mišic, jeter, mlinčka in srca. Vzorce smo preiskali z mikrobiološko metodo s testnim mikroorganizmom Bacillus stearothermophillus var. calidolactis C 953 in z metodo štirih plošč. Metoda štirih plošč je bila občutljivejša na ostanke salinomicina kot metoda z Bac. stearothermophillus. Ostanke salinomicina smo ugotovili v tkivih piščancev od 4. do 6. dneva po eksperimentalnem krmljenju.
Deskriptorji		MEAT FOOD MICROBIOLOGY COCCIDIOSTATS DRUG RESIDUES BACILLUS STEAROTHERMOPHILUS CHICKENS