Avtor/Urednik     Mueller-Premru, M
Naslov     Značilnosti in vloga koagulaza negativnih stafilokokov kot povzročiteljev kateterskih seps
Tip     monografija
Kraj izdaje     Ljubljana
Založnik     Medicinska fakulteta
Leto izdaje     1991
Obseg     str. 82
Jezik     slo
Abstrakt     In this study the microbiological characteristics of ciagulase-negative staphylococci isolated from blood and catheters of patients in a neonatal intensive care unit were investigated. From 33 patients in this study 41 strains of coagulase-negative staphylococci were solated. The specimens were collected from October 1988 until October 1990. From 8 of 33 patients in this study simultaneous isolates of coagulase-negative staphylococci from blood and catheters were obtained. In other patients coagulase-negative staphylococci were isolated from blood or catheter only. Identification of coagulase-negative staphylococci to the species level was done according to a modified scheme of Hloos and Schleifer. Quantitative adherence was studied by the Christensen's method. The modified Christensen's method of quantitative adherence was introduced for blood and catheter strains of coagulase-negative staphylococci. We found that blood and catheter S. epidermitis strains were more adherent that saprophytic skin strains from the control group of young and healthy people. Strains of S. epidermitis from blood and catheters were also more adherent than strains of the other coagulase-negative species from the same sites. The highest quantitative adherence values were obtained from S. epidermidis strains of patients, who were suspected to have catheter-related sepsis. More adherent than nonadherent strains of S. epidermidis were multiresistant. A correlation existed between the patient's clinical picture and the level of adherence. Epidemiological typing of isolates was also performed. All of the S. epidermidis strains belonged to 4 biotypes. Isolates from patients who were suspected to have catheter related sepsis were of the same biotype and had the same antibiograms. Plasmid profiles were done for ten S. epidermidis isolates. Only 2 strains had detectable plasmids of different sizes. Until now we have not been able to prove the identify of more isolates with this method.
Deskriptorji     CATHETERIZATION
SEPTICEMIA
STAPHYLOCOCCUS
COAGULASE
INTENSIVE CARE UNITS, PEDIATRIC
CROSS INFECTION
MICROBIAL SENSITIVITY TESTS
BACTERIAL TYPING TECHNICS
CHILD