Avtor/Urednik		Vilhar, B; Vidic, T; Jogan, N; Dermastia, M
Naslov		Genome size and the nucleolar number as estimators of ploidy level in Dactylis glomerata in the Slovenian Alps
Tip		članek
Vir		Plant Syst Evol
Vol. in št.		Letnik 234
Leto izdaje		2002
Obseg		str. 1-13
Jezik		eng
Abstrakt		We studied five natural populations of Dactylis glomerata L. (Poaceae) growing at different altitudes in the south-eastern fringe of the Alps in northern Slovenia to determine the subspecies. The stomatal length, the pollen diameter and chromosome counts were consistent with the tetraploid taxon D. glomerata subsp. glomerata (2n = 4x = 28). Genome size was measured in 55 individuals. The mean 2C value was 8:6 pg DNA. The mean 2C values of populations growing at different altitudes showed only 2.1 % variation, and no correlation was observed between altitude and genome size. In D. glvrnerata subsp. glomerata eight nucleoli were observed in late telophase, indicating that the nucleolus-organising regions inherited from both diploid parent species are functional. We demonstrate that both genome size and the number of nucleoli may be used to determine the ploidy level as an alternative to chromosome counting.
Deskriptorji		PLANTS ECOSYSTEM GENOME, PLANT PLOIDIES KARYOTYPING IMAGE CYTOMETRY SILVER STAINING NUCLEOLUS ORGANIZER REGION CELL NUCLEOLUS TELOPHASE