Avtor/Urednik		Gregorčič, Sergeja; Jan, Matevž
Naslov		Aktivacija molekul CD69 in CD25 na za antigene bakterije Borrelia afzelli specifičnih CD4+, CD8+ in CD19+ limfocitnih bolnikov z erythema migrans
Tip		monografija
Kraj izdaje		Ljubljana
Založnik		Medicinska fakulteta
Leto izdaje		2002
Obseg		str. 40
Jezik		slo
Abstrakt		BACKGROUND: Lyme borreliosis is endemic in Slovenia. It is a disease caused by Borrelia burgdorferi sensu lato and is transmitted by ticks. The most frequently isolated borrelia from skin manifestations of patients with borreliosis in Slovenia is B. afzelii. Diagnosis of borreliosis is based on clinical and laboratory findings. Laboratory diagnosis is aimed toward detecting the presence of antibodies specific to borrelial antigens. Sometimes isolation of borrelia is used. Humoral immune response is usually poor or absent in cases of early localized borreliosis. We assume that in the early phase of borreliosis the presence and the activity of the disease could be detected by measuring cellular immune response. AIM: We measured the presence of CD25 and CD69 molecules that express on surfaces of activated lymphocytes. We also reflected the possibility of transfering our research method to laboratory diagnostics of early localized borreliosis. HYPOTHESIS: We expected that after in vitro stimulation with borrelial antigens patients with erythema migrans will have higher proportion of lymphocytes expressing activation markers CD25 and CD69 than healthy individuals, which were not infected with borrelia. METHODS: Whole blood of nine patients with erythema migrans and seven healthy induviduals was used in the research. Whole blood was incubated with antigens of B. afzelii for 24 hours and then we measured the expression of activation markers CD25 and CD69 on surfaces of different populations and subpopulations of lymphocytes using flow cytometry. We also measured IFN-gamma concentration in the plasma remnant after in vitro stimulation of lymphocytes with borrelial antigens. Patients had their titre of B. burgdorferi sensu lato specific antibodies measured. All results were statistically evaluated using Student's t-test. Correlation was expressed as Pearson's correlation coefficient (r). (Abstract truncated at 2000 characters).
Deskriptorji		ERYTHEMA CHRONICUM MIGRANS BORRELIA ANTIGENS, BACTERIAL ANTIGENS, CD4 ANTIGENS, CD8 ANTIGENS, CD19 RECEPTORS, INTERLEUKIN-2 INTERFERON TYPE II FLOW CYTOMETRY LYMPHOCYTES