Avtor/Urednik		Štrus, Petra; Triller, Katja
Naslov		Neposredno dokazovanje in genotipizacija povzročitelja bolezni mačje opraskanine
Tip		monografija
Kraj izdaje		Ljubljana
Založnik		Medicinska fakulteta
Leto izdaje		2002
Obseg		str. 4250
Jezik		slo
Abstrakt		BACKGROUND. Cat-scratch disease is one of the causes of clinically evident lymphadenopathy. Early recognition of the disease is crucial for further diagnostic and therapeutic investigations. The disease is considered zoonosis and the cats are a big natural reservoir. Since children come in contact with cats very frequently, the cat-scratch disease is most common in this age group. The causative agent is a small bacillus, Bartonella henselae, which is considered an emerging pathogen. In the case of Bartonella, the classical diagnostic methods are time-consuming and unreliable. To establish a precise diagnosis of infection (to the species or possibly genotype level), newer, molecular methods of diagnosis are applied. AIM. There have not been any studies in Slovenia yet, which could confirm the presence of particular species or genotypes of Bartonella in patients with cat-scratch disease. The aim of the present study was to fmd out, which bacterial species or genotypes are present in lymph nodes of patients with clinically manifested disease. Two different parts of the genome were analysed (a part of the htrA gene and intergenic spacer region) for inter- and intra- species differentiation of genus Bartonella. HYPOTHESIS. Based on the various studies in Europe and USA we expect to isolate species Bartonella henselae from lymph node specimens of patients with clinical signs of catscratch disease. With polymerase chain reaction and sequence analysis, we further expect to determine the genotypes of Bartonella henselae. METHODS. Our study included 24 randomly chosen samples, taken from 21 different patients. The samples were mostly lymph node specimens, but also blood and cerebrospinal fluid. After DNA extraction, part of the htrA gene and ITS intergenic spacer were amplified by polymerase chain reaction. The nucleotide sequences of the amplified parts were determined and compared with those in GenBank to establish the genotype. (Abstract truncated at 2000 characters).
Deskriptorji		CAT-SCRATCH DISEASE BARTONELLA HENSELAE LYMPH NODES POLYMERASE CHAIN REACTION BASE SEQUENCE GENOTYPE GENE LIBRARY