Avtor/Urednik | Štempelj, M; Čarman-Kržan, M; Ferjan, I | |
Naslov | Regulatory role of extracellular Na+ and Ca+ ions in nerve growth factor induced histamine secretion from rat mast cells | |
Tip | članek | |
Vir | Inflamm Res | |
Vol. in št. | Letnik 52 | |
Leto izdaje | 2003 | |
Obseg | str. 74-8 | |
Jezik | eng | |
Abstrakt | Objective and design: This study was aimed to investigatc effccts of extracellular Na+ and Ca2+ ions on ncrvc growth factor (NGF) induced histamine release from mast cells. Material: Isolated peritoneal mast cells were obtained from male Wistar rats. Methods: Cells were suspended in solution with different concentration of Na+ and Ca2+ ions and stimulated with NGF Histamine release was assayed spectrofluorometrically. Results: NGF (0.001-1 microg/ml) dose-dependently releases histamine from mast cell at physiological extracellular Na' (134 mM) and Ca2+ (1mM) conditions. Lowering extracellular Ca2+ ions to 0.1 mM reduced histamine response to nearly basal level. However, the removal of extracellular Na+ ions significantly enhanced the secretion provoked by NGF (0.6 microg/ml) in low Ca2+ medium. Amiloride, an inhibitor of Na+/Ca2+ and Na+/H+ exchangers inhibited the potentiating effect of sodium free conditions. Conclusions: Our results suggest that the activity of Na+/Ca2+ and/or Na+/H+ exchange mechanisms could be of particular importance in the secretory process of mast cells induced by NGF. | |
Deskriptorji | MAST CELLS SODIUM CALCIUM NERVE GROWTH FACTORS HISTAMINE RELEASE RATS, WISTAR AMILORIDE |