Avtor/Urednik | Potokar, M; Milisav, I; Kreft, M; Stenovec, M; Zorec, R | |
Naslov | Apoptosis triggered redistribution of caspase-9 from cytoplasm to mitochondria | |
Tip | članek | |
Vir | Febs Lett | |
Vol. in št. | Letnik 544, št. 1-3 | |
Leto izdaje | 2003 | |
Obseg | str. 153-9 | |
Jezik | eng | |
Abstrakt | Caspase-9 is an apoptosis initiator protease activated as a response to the mitochondrial damage in the cytoplasmic complex apoptosome. By fluorescence labelling of proteins, confocal microscopy and subcellular fractionations we demonstrate that caspase-9 is in the cytoplasm of non-apoptotic pituitary cells. The activation of apoptosis with rotenone triggers the redistribution of caspase-9 to mitochondria. Experiments using the general caspase inhibitor z-VAD.fmk and the specific caspase-9 inhibitor z-LEHD.fmk show that the caspase-9 redistribution is a regulated process and requires the activity of a caspase other than the caspase-9. We propose that this spatial regulation is required to control the activity of caspase-9. | |
Deskriptorji | PITUITARY GLAND APOPTOSIS TRANSFECTION CYSTEINE PROTEINASES MICROSCOPY, CONFOCAL IMMUNOHISTOCHEMISTRY POLYMERASE CHAIN REACTION |