Avtor/Urednik		Kotnik, Vladimir; Ruprecht, Ruth Rebeka; Mozetič-Francky, Bojana; Muzlovič, Igor; Čurin-Šerbec, Vladka
Naslov		MIF pri bolnikih s kliničnim sumom na sepso
Prevedeni naslov		Macrophage migration inhibitory factor in patients with clinically suspected sepsis
Tip		članek
Vir		In: Baklan Z, Reberšek-Gorišek J, Kotnik-Kervokljan B, editors. Zbornik predavanj in praktikum Bedjaničev simpozij z mednarodno udeležbo o temi Sepsa in septični šok; 2003 maj 31-31; Maribor. Maribor: Splošna bolnišnica Maribor,
Leto izdaje		2003
Obseg		str. 79-87
Jezik		slo
Abstrakt		The sepsis syndrome is usually induced by microbial pathogens and reflects excessive stimulation of the processes of innate immunity. Bacterial components activate complex cellular and humoral response resulting in the release of excessive amounts of inflammation mediators. Direct activation of complement system is expected as well as production of inflammatory cytokines. MIF seems to be an important mediator of septic shock: lts mechanism is incompletely understood, but its involvement in immunity and disease is more and more elucidated. MIF is secreted by anterior pituitary cells in response to LPS stimulation. It was shown that this cytokine has an important role in post-acute phase of endotoxaemia. Recombinant murine MIF greatly enhances lethality in experimental models of septic shock and anti-MIF antibodies has a full protection against endotoxaemia. To investigate the role of MIF as a marker of systemic inflammation blood was collected from 9 patients hospitalized because of clinically suspicious sepsis. Blood was taken every 4 hours to get the information on the dynamics of MIF appearance. CRP, PCT levels and complement activity were measured in the samples also to get better insight in the mechanism of the disease. The MIF specific ELISA was performed on the samples using our own monoclonal anti-MIF antibodies. Markedly increased plasma concentrations of MIF were measured in patients with clinically evident sepsis in comparison to healthy controls.
Deskriptorji		SEPSIS MACROPHAGE MIGRATION-INHIBITORY FACTORS CYTOKINES COMPLEMENT PATHWAY, CLASSICAL COMPLEMENT 5A COMPLEMENT 3D PROSPECTIVE STUDIES