Avtor/Urednik		Salimović, Irma
Naslov		Humani virusi papiloma pri slovenskih bolnicah s ponavljajočimi Pap II rezultati presejalnega testa po Papanicolaou
Tip		monografija
Kraj izdaje		Ljubljana
Založnik		Medicinska fakulteta
Leto izdaje		2003
Obseg		str. 88
Jezik		slo
Abstrakt		Background. A persistent infection with high-risk genotypes of human papilloma viruses (HPV) represents the most important etioiogic factor for the development of cervical cancer, the second most frequent cancer in women in Slovenia as well as elsewhere in the world. !n the detection of precancerous lesions the cervical Papanicolaou (Pap) smear screening is used in Slovenia and worldwide. Management of patients with repeat abnormal smears (Pap II) represents a great and complex clinical and public health probiem; repeat cytologic examinations are the routine procedure in many countries, also in Slovenia, although the sensitivity of Pap smear testing in the detection of cervical intraepithelial neoplasia (CIN) II and III is relatively low. ln cases of abnormal squamous cells and mildly dyskaryotic cells the presence of infections with high-risk HPV genotypes is being increasingly used as a complementary method to Pap smear testing. Matherial and methods. In the study we enrolled 148 cervical samples of women who within two years had three subsequent Pap II smears (abnormal squamous cells and mildly dyskaryotic cells). The prevalence of HPV infections was determined using three molecular tests: Hybrid Capture II (HCII) (Digene Corporation, Gaithersburg, USA) and two variants of polymerase chain reaction (PCR-PGMY11/PGMY09 and PCR-CPI/CPIIG). HPV genotypes were determined using the method of enzyme restriction of PCR products amplified by groupspecific oligonucleotide primers PGMY11/PGMY09. (Abstract truncated at 2000 characters)
Deskriptorji		CERVIX NEOPLASMS CERVICAL INTRAEPITHELIAL NEOPLASIA VAGINAL SMEARS PAPILLOMAVIRUS, HUMAN GENOTYPE POLYMERASE CHAIN REACTION IN SITU HYBRIDIZATION SLOVENIA