Avtor/Urednik		Škof, Anita
Naslov		Dokazovanje prisotnosti bakterij Staphylococcus aureus in Pseudomonas aeruginosa v zdravilih z verižno reakcijo s polimerazo
Prevedeni naslov		Staphylococcus aurerus and Pseudomonas aeruginosa in medicaments by polymerase chain reaction
Tip		monografija
Kraj izdaje		Ljubljana
Založnik		Medicinska fakulteta
Leto izdaje		2002
Obseg		str. 94
Jezik		slo
Abstrakt		Microbiological quality assurance of pharmaceutical products is important part of technological process from two reasons: stability of products and safety for users. The European Pharmacopoeia prescribes maximal acceptable total viable aerobic count per gram or milliliter of product and absence of certain types of microorganisms which can be considered a hazard to users. In medicaments for topical use the absence of Staphylococcus aureus and Pseudomonas aeruginosa is required. Microbial contamination by both of microorganisms is normally low, therefore sample enrichment must be performed to effectively recover these bacterial pathogens from pharmaceutical products. For isolation and identification of both bacteria by standard microbiological method 5 to 7 days are required. Therefore there is a need to develop and apply new technologies, that will be more rapid and sensitive. The purpose of this work was to develop a PCR assay for faster detection of low levels of Staphylococcus aureus and Pseudomonas aeruginosa in medicaments for topical use. Sample pre-enrichment was used to improve the sensitivity of method. After 24-hour enrichment bacterial DNA was isolated with the High Pure PCR Template Preparation Kit (Roche). Selected target sequence of bacterial genome was detected by a real-time PCR. The PCR assay was performed in a LightCyclerTM, which enables simultaneous amplification of target sequence and detection of PCR product with hybridization probes. (Abstract truncated at 2000 characters)
Deskriptorji		DRUG CONTAMINATION STAPHYLOCOCCUS AUREUS PSEUDOMONAS AERUGINOSA POLYMERASE CHAIN REACTION