Avtor/Urednik     Zheng, Yong-Hui; Plemenitaš, Ana; Fielding, Christopher J; Peterlin, Matija B
Naslov     Nef increases the synthesis of and transports cholesterol to lipid rafts and HIV-1 progeny virions
Tip     članek
Vir     Proc Natl Acad Sci U S A
Vol. in št.     Letnik 100, št. 14
Leto izdaje     2003
Obseg     str. 8460-5
Jezik     eng
Abstrakt     HIV buds from lipid rafts and requires cholesterol for its egress from and entry into cells. Viral accessory protein Nef plays a major role in this process. In this study, it not only increased the biosynthesis of lipid rafts and viral particles with newly synthesized cholesterol, but also enriched them. Furthermore, via the consensus cholesterol recognition motif at its C terminus, Nef bound cholesterol. When this sequence was mutated, Nef became unable to transport newly synthesized cholesterol into lipid rafts and viral particles. Interestingly, although its levels in lipid rafts were not affected, this mutant Nef protein was poorly incorporated into viral particles, and viral infedivity decreased dramatically. Thus, Nef also transports newly synthesized cholesterol to the site of viral budding. As such, it provides essential building blocks for the formation of viruses that replicate optimally in the host.
Deskriptorji     CHOLESTEROL
GENE PRODUCTS, NEF
HIV-1
VIRION
BIOLOGICAL TRANSPORT
CHO CELLS
CELL LINE
CRICETULUS
CYTOCHROME P-450
ENZYME INDUCTION
GENES, REPORTER
HAMSTERS
JURKAT CELLS
KIDNEY
LANOSTEROL
MEVALONIC ACID
MODELS, BIOLOGICAL
VIRUS REPLICATION
RECOMBINANT FUSION PROTEINS
PROTEIN BINDING
PROMOTER REGIONS (GENETICS)
OXIDOREDUCTASES