Avtor/Urednik		Kojc, Nika
Naslov		Histološke in imunohistokemične značilnosti stromalnih celic pri epitelijskih hiperplastičnih spremembah in ploščatoceličnem karcinomu grla in žrela
Tip		monografija
Kraj izdaje		Ljubljana
Založnik		Medicinska fakulteta
Leto izdaje		2003
Obseg		str. 55
Jezik		slo
Abstrakt		Recent studies have shown that stromal reaction in cancer has a more important diagnostic and prognostic significance than previously thought. The aim of our study was therefore to analyze the distribution and immunohistochemical phenotype of stromal cells in epithelial hyperplastic lesions (EHL) and squamous carcinoma (SC) of the larynx and pharynx, and to establish their diagnostic and prognostic significance. We investigated samples of 42 resected larynxes with SC, 12 laryngeal biopsies of EHL and SC and 6 samples of normal adult and fetal laryngeal mucosa. Immunohistochemistry was performed using antibodies against a-smooth muscle actin (SMA), CD34, CD31, CD117, TGF(31 and TGFbeta1 receptor. RNA in situ hybridization was performed using specific oligonucleotides for TGFbeta1. The stroma of EHL contained scattered CD34-positive cells, but there were no SMA-positive myofibroblasts. In contrast, the stroma of SC contained SMA-positive myofibroblasts but there were no CD34-positive cells. Some myofibroblasts stained for CD117. TGFbeta1 and its receptor were present in tumor cells as well as in inflammatory cells and myofibroblasts in the stroma of SC. Stromal reaction at the invasive front and in peritumoral space was dependent of the pattern of tumor growth at the invasive front (infiltrative, expansive, mixed). Only association between the intensity of inflammatory reaction in the peritumoral zone and the tumor diameter was statistically significant (p<0.05). Our results show that disappearanec of CD34-positive cells and appearance of SMA-positive myofibroblasts in the stroma is associated with transformation of EHL to SC of the larynx and pharynx. (Abstract truncated at 2000 characters).
Deskriptorji		LARYNGEAL NEOPLASMS PHARYNGEAL NEOPLASMS STROMAL CELLS CARCINOMA, SQUAMOUS CELL NEOPLASM INVASIVENESS NEOPLASM STAGING IMMUNOHISTOCHEMISTRY HYPERPLASIA IN SITU HYBRIDIZATION, FLUORESCENCE ANTIGENS, CD34 MICROFILAMENTS MUSCLE, SMOOTH PROTO-ONCOGENE PROTEINS C-KIT TRANSFORMING GROWTH FACTOR BETA PROGNOSIS