Author/Editor		Zeppa, Pio; Marino, Gilda; Troncone, Giancarlo; Fulciniti, Franco; De Renzo, Amalia; Picard, Marco; Benincasa, Giulio; Rotoli, Bruno; Vetrani, Antonio; Palombini, Lucio
Title		Fine-needle cytology amd flow-cytometry immunophenotyping and sub-classification of non-Hodgkin lymphoma
Type		članek
Source		In: Vizjak A, Ferluga D, Bussolati G, editors. Update in pathology. Proceedings of the 19th European congress of pathology: nephropathology pre-congress meeting advances in nephrology, pulmonary pathology pre-congress meeting; 2003 Sep 6-11; Ljubljana. Ljubljana: Faculty of medicine,
Publication year		2003
Volume		str. 46-8
Language		eng
Abstract		Flow cytometry (FC) is a useful adjunct to %neneedle aspiration cytology (FNC) in evaluating lymphoproliferative disorders. We present a series of 307 lymph nodal , and extra lymph nodal lymphoproliferative disorders diag- i nosed by FNC and FC. FC was performed over a four-year period on 185 palpable and 122 impalpable lymph nodal and extra lymph-nodal lymphoproliferative processes under US or CT control. FC was performed using the following fluoresceinated antibodies: CD3, CD4/8, CDS, CD19kappa/lambda, FMC7/CD23/CD19, CD2/3/7, CD38/56/19, Bcl-2, CD13/HLA-DR. The series comprised 15 inadequate, 10 suspect and 135 benign reactive hyperplasias (BHR), 70 primary non Hodgkin lymphomas (NHL) and 77 relapses of NHL (rNHL). FC/FNC diagnoses were controlled by clinical follow-up and/or histology when performed. FC applied to FNC enhances the precision of cytological diagnosis in lymph-nodal and extra-lymphnodal lymphoproliferative disorders and allows sub classification in more than half of the cases, avoiding invasive surgical biopsies in many patients.
Descriptors		LYMPHOMA, NON-HODGKIN'S BIOPSY, NEEDLE FLOW CYTOMETRY IMMUNOPHENOTYPING