Author/Editor		Vollmer, E; Sen-Gupta, R; Galle, J; Goldmann, T
Title		Methods and techniques in routine and research of lung pathology
Type		članek
Source		In: Vizjak A, Ferluga D, Bussolati G, editors. Update in pathology. Proceedings of the 19th European congress of pathology: nephropathology pre-congress meeting advances in nephrology, pulmonary pathology pre-congress meeting; 2003 Sep 6-11; Ljubljana. Ljubljana: Faculty of medicine,
Publication year		2003
Volume		str. 354-7
Language		eng
Abstract		Besides giving a rough overview about the state of the art in lung pathology, this review likes to focus upon some highlighted aspects from our lung specific work during the last years. Immunohistochemical markers for differential diagnostics of malignancies in the lungs are discussed. Within the growing field of molecular pathology we also describe the possibilities and limitations of PCR for the detection of mycobacteria in formalin-fixed, paraffmembedded tissues. The growing demand for the detection of prognostic and diagnostic biomarkers by application of molecular biological techniques has been slowed down to a large degree by the use of formalin and its influence onto antigenic structures and nucleic acids, although other reasons also exist. With regard to morphology a limitation in the possibilities, compared to frozen sections, takes place. With the introduction of the HOPE (Hepes glutamic acid buffer mediated Organic solvent Protection Effect)-fixation to date both has become possible; substantially enlarged possibilities concerning the detection of DNA, RNA and proteins in combination with excellent morphological results comparable to formalin-fixed tissues.
Descriptors		LUNG NEOPLASMS IMMUNOHISTOCHEMISTRY POLYMERASE CHAIN REACTION