Author/Editor     Gašperšič, Nataša
Title     Aneksin V, kofaktor za antifosfolipidna protitelesa in njegova vloga pri bolnikih z ishemično možganskožilno boleznijo
Type     monografija
Place     Ljubljana
Publisher     Medicinska fakulteta
Publication year     2003
Volume     str. 61
Language     slo
Abstract     Antiphospholipid antibodies (aPL) are a heterogeneous group of antibodies associated with an increased risk for thromboses and pregnancy complications. They recognise epitopes on phospholipid binding plasma proteins of which beta2-glycoprotein I (beta2GPI) is the most studied. One among possible cofactors for aPL is also annexin V (ANXV). In the previous studies antibodies against ANXV (aANXV) were detected in the sera of different patients, mainly those with a particular systemic autoimmune disease. The association between aANXV and thrombotic complications, as well the pathogenetic mechanism of their action still remains unclear. In our study the role of aANXV was focused. For the detection of aANXV in the sera samples we used enzyme linked immunosorbent assay (ELISA). The method has not been standardised yet, therefore we thoroughly tested and optimised each step of the assay before testing the patients' sera. Forty patients at the age no more than 40 years who experienced a cerebrovascular thrombotic event were selected for the study. After clinical re-examination at least two blood samples from each patient were obtained eight weeks apart and sera were analysed using ELISA for the presence of aANXV and antibodies against cardiolipin, beta2GPI and prothrombin. Elevated levels of aANXV were present in 3/40 (8%) patients with a history of a cerebrovascular event. Two female patients had only IgG aANXV among all tested antibodies. Antibodies against cardiolipin were detected in 4/40 (10%) patients, 2/40 (5%) had antibodies against 132GPI and 1/40 (3%) against prothrombin. aPL positive patients also had classical risk factors for a cerebrovascular disease such as smoking, elevated blood pressure, elevated serum cholesterol or obesity. (Abstract truncated at 2000 characters).
Descriptors     CEREBROVASCULAR DISORDERS
ANTIBODIES, ANTIPHOSPHOLIPID
ANTIBODIES, ANTICARDIOLIPIN
ADULT
IGG
IGM
IGA
CEREBRAL ISCHEMIA
ENZYME-LINKED IMMUNOSORBENT ASSAY
PHOSPHOLIPIDS
GLYCOPROTEINS
MICROSCOPY, FLUORESCENCE