Author/Editor		Tepeš, Bojan
Title		Primerjava dveh ureaznih bioptičnih testov za dokaz okužbe s Helicobacter pylori pred antimikrobnim zdravljenjem in po njem
Translated title		Prospective comparison of two rapid urease tests for the diagnosis of Helicobacter pylori before and after antimicrobial treatment
Type		članek
Source		Zdrav Vestn
Vol. and No.		Letnik 72, št. 9
Publication year		2003
Volume		str. 503-5
Language		slo
Abstract		Background. Rapid urease bioptic test (RUT) is a basic test for detection of Helicobacter pylori at upper gastrointestinal endoscopies. There are different RUT tests available commercially and the main difference between them lies in their reaction time. In every day clinical practice it is important for the physician to prescribe treatment at the time of upper gastrointestinal endoscopy. Methods. This study presents comparison of two different RUTs (Pliva bioptat(e) and CLO) in terms of their accuracy and needed reaction time for the test to become positive. Fifty duodenal ulcer patients with Helicobacter pylori infection were included. All of them were tested with two rapid urease tests, histology, culture and 13C urea breath test. Results. Sensitivity and specificity of both RUTs were very high and without any difference between them. Statistically significant difference appeared in the form of time needed for the test to become positive. Pliva bioptat(e) test became positive after 30 minutes in 48/50 (96%) of patients before introduction of the antimicrobial treatment compared with 32/50 (64%) of patients tested with the CLO test (p < 0.05). A month after antimicrobial therapy Pliva bioptat(e) test was positive in 9/11 (81%) after 30 minutes, compared with 3/11 (27%) of patients with the CLO test (p < 0.05). Conclusions. Pliva bioptate test has practical advantages for physicians (endoscopists) who need a rapid and accurate method of diagnosing of Helicobacter pylori infection before and after antimicrobial therapy.
Summary		Izhodišča. Hitri ureazni bioptični test (HUT) je osnovni diagnostični test za ugotavljanje okužbe s Helicobacter pylori pri endoskopskih preiskavah zgornjih prebavil. Razlike med posameznimi HUT testi so predvsem v hitrosti dobljenega izvida, kar je klinično pomembno. Želeno je, da se bolniku prisotnost okužbe in s tem tudi planirano zdravljenje lahko predpiše ob zaključku endoskopske preiskave. Metode. V študiji sem primerjal hitrost pozitivne reakcije in natančnost dveh ureaznih bioptičnih testov: CLO in Pliva bioptat V študijo je bilo vključenih 50 bolnikov s Helicobacter pylori pozitivno ulkusno boleznijo. Kot diagnostične teste smo uporabili dva HUT testa, histološko preiskavo, kulturo in 13C urea dihalni test. Rezultati. Senzitivnost in specifičnost obeh testov pred zdravjenjem in po njem je bila velika in se med testoma ni razlikovala tako pred zdravljenjem kot tudi po njem. Statistično značilna razlika pa je nastopila v hitrosti pozitivne reakcije pred zdravljenjem in po njem v korist Pliva bioptat testa. Pliva bioptat je bil pozitiven po 30 minutah pri 48/50 (95% bolnikov) pred uvedbo antimikrobnega zdravljenja v primerjavi z 32/50 (64% bolnikov) pri CLO testu (p < p,05). Mesec dni po končanem antimikrobnem zdravljenju je bil Pliva bioptat test pozitiven pri 9/11 bolnikov (81%) po 30 minutah v primerjavi z le 3/12 (27%) pri CLO testu (p < 0,05). Zaključki. Pliva bioptat hitri ureazni test je zanesljiv in hiter test za dokaz okužbe s Helicobacter pylori pred antimikrobnim zdravljenjem okužbe in po njej.
Descriptors		STOMACH ULCER HELICOBACTER PYLORI UREASE SENSITIVITY AND SPECIFICITY