Author/Editor		Bresjanac, Mara; Šmid, Lojze M; Vovko, Tomaž D; Petrič, Andrej; Barrio, Jorge R; Popović, Mara
Title		Molecular-imaging probe-2-(1-(6-((2-fluoroethyl)(mathyl)amino)-2-naphthyl)ethylidene) malononitrile labels prion plaques in vitro
Type		članek
Source		J Neurosci
Vol. and No.		Letnik 23, št. 22
Publication year		2003
Volume		str. 8029-33
Language		eng
Abstract		The study aimed to evaluate the fluorescent molecular-imaging probe 2-(1-{6-[(2-fluoroethyl)(methyl)amino]-2-naphthyl}ethylidene)mal ononitrile (FDDNP) for its ability to selectively and reproducibly label prion plaques in fixed, paraffin-embedded cerebellar sections from patients of confirmed Gerstmann-Straussler-Scheinker disease, sporadic Creutzfeldt-Jacob disease (CJD) with kuru plaques, and variant CJD (vCJD). FDDNP is a highly hydrophobic, viscosity-sensitive, solvent-sensitive, fluorescent substance, whose radiofluorinated analog (18F)FDDNP has recently been successfully used to label senile plaques and neurofibrillary tangles in the living brain of Alzheimer's disease patients with positron emission tomography. Our results show that FDDNP reliably identifies all prion plaques, including small cluster-plaques in vCJD. This finding may open new in vivo diagnostic possibilities for vCJD.
Descriptors		PRION DISEASES NITRILES MOLECULAR PROBES MOLECULAR PROBE TECHNIQUES FLUORESCENT DYES SUBSTRATE SPECIFICITY SENSITIVITY AND SPECIFICITY REPRODUCIBILITY OF RESULTS IMMUNOHISTOCHEMISTRY GERSTMANN-STRAUSSLER SYNDROME CREUTZFELDT-JAKOB SYNDROME CEREBELLUM