| Author/Editor | Bunc, Gorazd; Kovačič, Srečko; Strnad, Simona | |
| Title | The influence of noradrenergic blockade on vasospasm and the quantity of cerebral dopamine beta-hydroxylase following subarachnoid haemorrhage in rabbits | |
| Type | članek | |
| Source | Wien Klin Wochenschr | |
| Vol. and No. | Letnik 115, št. 17-18 | |
| Publication year | 2003 | |
| Volume | str. 652-9 | |
| Language | eng | |
| Abstract | Background: The aim of this study of experimental subarachnoid haemorrhage (SAH) and exclusion of the sympathetic nervous system (SNS) in rabbits was to find out if changes in the central noradrenergic areas of the hypothalamus and brain stem could be ascertained, in parallel with measurement of the intensity of chronic cerebral vasospasm in the basilar arteries. Mefhods: Histologic specimens were prepared by perfusion fixation on day 8 after the SAH. The spastic effect of experimentally induced SAH in New Zealand rabbits was investigated: firstly, using our previously developed method for measuring the corrugation coefficient (CC) of the vessel intima on precisely defined locations of the basilar artery (BA) with the aid of computer image analysis; and secondly, by immunohistochemical assessment of the concentration and localization of dopamine [3-hydroxylase (DBH), using anti-DBH, at precisely defined sites of the hypothalamus and brain stem of the same rabbit. Results: The intima of the BA, assessed by CC, was significantly less corrugated and had significantly less DBH in group A (the control group without SAH and without additional interventions; mean CC = 1.192, P = 0.004; median DBH = 0.50, P = 0.044), in group C (SAH and a-blocker phenoxybenzamine; mean CC = 1.142, P = 0.000; median DBH = 0.75, P = 0.001), and in group D (SAH and cervical gangliectomy; mean CC = 1.210, P = 0.003; median DBH = 0.50, P = 0.002) compared with group B (rabbits with SAH and without medication). Group B showed a significantly more intensive accumulation of DBH (median DBH = 1.15) and, according to the CC (mean CC = 1.369), more intensive corrugation of the intima of BA than all other groups. The correlation between CC and DBH for all the rabbits (groups A, B, C and D together) was significantly positive (Spearman Rho = 0.470; p = 0.010). (Abstract truncated at 2000 characters). | |
| Descriptors | SUBARACHNOID HEMORRHAGE BASILAR ARTERY TUNICA INTIMA IMAGE PROCESSING, COMPUTER-ASSISTED RABBITS IMMUNOHISTOCHEMISTRY DOPAMINE BETA-HYDROXYLASE HYPOTHALAMUS BRAIN STEM CEREBRAL ISCHEMIA, TRANSIENT PHENOXYBENZAMINE DISEASE MODELS, ANIMAL |