Author/Editor		Klančnik, Anja; Skočir, Petra; Smole-Možina, Sonja
Title		Hladni šok ter stradanje vplivajo na morfologijo, kultivabilnost in preživelost bakterij Campylobacter jejuni
Translated title		Cold shock and starvation influence viability, culturability and morphology of Campylobacter jejuni
Type		članek
Source		In: Dobeic M, Vudrag M, Berger T, editors. Zbornik referatov 1. interdisciplinarni simpozij "DDD, zdravje in okolje" z mednarodno udeležbo; 2003 okt 17-18; Ljubljana. Ljubljana: Zavod za zdravstveno varstvo,
Publication year		2003
Volume		str. 195-202
Language		slo
Abstract		Campylobacter jejuni is a leading cause of acute bacterial foodborne gastroenteritis worldwide. Its physiology is in many senses unusual. It seems to lack many of adaptive responses to environmental stresses known in other foodborne pathogens. However, under stress conditions campylobacters transit from a spiral-shaped to the more resistant coccoid form, usually accompanied by a transformance into a viable but nonculturable state (VBNC). More research is needed to elucidate the importance of this phenomenon for survival of pathogenic campylobacters during food production chain. This was the reason for our investigation of transition of C. jejuni actively growing and stationary phase cells into a VBNC and coccoid form following their viability, culturability and cellular morphology after cold shock at 25degreesC. Acridine orange direct count (AODC), growth on Karmali agar as well as fluorescent and electron microscopy were methods used, respectively. We confirmed that low temperatures enhanced survival of C. jejuni cells for cca 20%, viability and culturability were preserved at least 30 days in rich medium (Preston) and more than 40 days in starved cells. It is remarkable that starved C. jejuni cells increased cold and heat resistance. The results indicate the absence of phenotypic stationary phase response of Campylobacter cells.
Descriptors		FOOD CONTAMINATION FOOD MICROBIOLOGY CAMPYLOBACTER JEJUNI TEMPERATURE STARVATION