Author/Editor		Lahdenne, Pekka; Pannelius, Jaana; Saxen, Harri; Heikkilae, Tero; Sillanpaeae, Heidi; Peltomaa, Mikka; Arnež, Maja; Huppertz, Hans-Iko; Seppaelae, Ilkka JT
Title		Improved serodiagnosis of erythema migrans using novel recombinant borrelial BBK32 antigens
Type		članek
Source		J Med Microbiol
Vol. and No.		Letnik 52
Publication year		2003
Volume		str. 563-7
Language		eng
Abstract		The performances of recombinant borrelial BBK32 proteins as antigens in the serology of erythema migrans (EM) were evaluated in an ELISA. Serum samples were obtained from 75 patients from different geographic areas where three borzelial species, Borrelia burgdorferi sensu stricto, Borrelia afzelii or Borrelia garinii, cause Lyme borreliosis. Antibodies to variant BBK32 proteins were compared with anti-flagella or with anti-IRs peptide antibodies. In IgG ELISA at presentation of EM, 65/75 (87%) patients had antibodies to one or more variants of BBK32, 29/75 (39%) had antibodies to flagella and 29/75 (39%) had antibodies to the VIsE IR6 peptide antigen. The immunoreactivity against variant BBK32 proteins differed in patients from different geographic regions. The present results suggest that the BBK32 proteins used in combination or in parallel may improve the laboratory diagnosis of EM.
Descriptors		ERYTHEMA CHRONICUM MIGRANS BORRELIA BURGDORFERI ANTIGENS, BACTERIAL BACTERIAL PROTEINS SEX FACTORS ADULT SERODIAGNOSIS SENSITIVITY AND SPECIFICITY RECOMBINANT PROTEINS ENZYME-LINKED IMMUNOSORBENT ASSAY ANTIBODIES, BACTERIAL