Author/Editor     Grach-Pogrebinsky, Olga; Sedmak, Bojan; Carmeli, Shmuel
Title     Protease inhibitors from a Slovenian lake Bled toxic waterbloom of the cyanobacterium Planktothrix rubescens
Type     članek
Source     Tetrahedron
Vol. and No.     Letnik 59
Publication year     2003
Volume     str. 8329-36
Language     eng
Abstract     Three new protease inhibitors, planktopeptin BL 1125, planktopeptin BL843 and planktopeptin BL 1061 were isolated, along with three known compounds, anabaenopeptin A, anabaenopeptin B and anabaenopeptin F from the hydrophilic extract of Planktothrix rubescens. The planar structure of the new compounds was determined by homonuclear and inverse-heteronuclear 2D NMR techniques as well as highresolution mass spectrometry. The absolute configuration of the asymmetric centers was studied using Marfey's method for HPLC and by comparison of the acid hydrolysate with authentic samples on a chiral HPLC column. The new peptides were found to be elastase and chymotrypsin inhibitors.
Descriptors     FRESH WATER
WATER POLLUTANTS
EUTROPHICATION
CYANOBACTERIA
PROTEASE INHIBITORS
SPECTRUM ANALYSIS, MASS
CHROMATOGRAPHY, HIGH PRESSURE LIQUID