Author/Editor		Radovanović, Mirjana
Title		Uporabnost hitre mikroskopske kvantitativne preiskave v napovedi nastanka sepse iz opeklinske rane
Type		monografija
Place		Ljubljana
Publisher		Medicinska fakulteta
Publication year		1991
Volume		str. 51
Language		slo
Abstract		The infection of the burn wound is one of major complications in treating patients with burns and thus it is important to know the present bacterial colonization. It must be analyzed with simple methods as soon as possible and the results obtained should be accurate. In this research we wanted to evaluate two methods of sampling clinical specimens and two microscopic methods of quantitative bacteriologic survey. Clinical samples from the same area of the burn wound were taken by the methods of biopsy and moist swab. For quantitative tests the following methods were used: the quantitative bacteriological culture technique - as reference method, microscopic acridineorange-staining, and Gram-staining techniques. The bacteria obtained through the reference method were identified by standard bacteriological procedures. The analyses of 50 moist swabs and 50 biopsies shows: * the method of biopsy remains the reference technique for clinical sampling - mixed pathogenic flora is more often found in biopsy samples and more pathogenic species of bacteria are isolated from them than they are from moist swab; * results of qualitative analyses correspond with data quoated in literature - Staphylococcus epidermidis, Staphylococcus aureus, Pseudomonas aeruginosa and Acinetobacter calcoaceticus anitratus are the most frequently isolated species; * the quantitative culture technique remains the best method for determining bacterial colonization in the burn wound - the results of microscopic methods have little correlation with those of the reference method, except in cases very different from the average values. On the other hand too many subjective and local factors make them less accurate, thus these methods can yield the bacterial growth only superficially. To designe a prophylactic chemotherapy scheme a clinic must also have the results of qualitative surveys, sensitivity tests of isolated bacteria, together with other clinical, microbiological and laboratory tests.
Descriptors		BURNS WOUND INFECTION SEPSIS BIOPSY BACTERIAL INFECTIONS