Author/Editor     Černe, Katarina
Title     Vsebnost trans in cis izomer maščobnih kislin v prehrani starostnikov v Sloveniji
Translated title     Trans and cis fatty acid isomers in food of elderly population in Slovenia
Type     monografija
Place     Ljubljana
Publisher     Biotehniška fakulteta
Publication year     1998
Volume     str. 99
Language     slo
Abstract     Purpose of the study was to analyse 97 samples of daily meals in homes of elderly people in R Slovenia, and sample of margarine Zvezda in order to determine their contents of trans and cis dietary octadecanoic (18:1) fatty acid isomers as relative ratios of oleic (9c-18:1) and elaidic (9t- 18:1) fatty acids to estimate the quality of fat from this point of view. This is the first estimate of trans and cis fatty acids intake in Slovenia. To overcame the metodologic difficulties in determining with precision and accuracy the content of trans and cis fatty acids in food samples we used two different methods: 1) gas chromatography with flame ionization detektor (GC-FID) (AOCS Official Method Ce 1c-89), 2) Ag-ion exchange liquid chromatography with ultraviolet detector (Ag-HPLC-UV. 89 samples contains trans 18:1 isomers (mean value 2,73% wtiwt) and 25 samples contains trans 18:2 isomers (mean value 0,21% wt/wt). Content of the trans 18:1 and trans 18:2 isomers could be considered low and probably represents a very low health risk. According to results for relative cis/trans 18:1 ratio values obtained with GC-FID 33 samples are in range 1-10, 29 samples are in range 10-20, 17 samples are in range 20-30, 10 samples are in range 30<. Lower relative cis/trans 18:1 isomers ratio means higher trans 18:1 content. The main contributor to the trans 18:1 intake is margarine (used in preparations of meals and less as a spread). Content of trans 18:1 isomers in margarine Zvezda is high (19,86 wt/wt} and relative cis/trans ratio value is 1,2. To lower trans 18:1 fatty aeids intake margarines with no trans or low in trans fatty acids are available on the S(ovenian market place if solid fat is needed.
Descriptors     HOMES FOR THE AGED
FOOD ANALYSIS
FATTY ACIDS, UNSATURATED
STEREOISOMERISM
AGED
MARGARINE
CHROMATOGRAPHY, HIGH PRESSURE LIQUID
CHROMATOGRAPHY, GAS