Author/Editor		Podgornik, Helena; Podgornik, Aleš
Title		Separation of manganese peroxidase isoenzymes on strong anion-exchange monolithic column using pH-salt gradient
Type		članek
Source		J Chromatogr B
Vol. and No.		Letnik 799
Publication year		2004
Volume		str. 343-7
Language		eng
Abstract		Different chromatographic methods including chromatofocusing are used for separation of manganese peroxidase (MnP) isoforms and their isolation from the fungal growth medium. We tested strong anion exchange methacrylate based monolithic columns as a stationary phase for fast separation of MnP's. Sodium acetate buffers of two different pH values (6 and 4) were used for formation of reproducible pH gradient. The entire cycle, involving analysis and column regeneration, was completed in 3 min. Use of pH gradient showed better MnP isoform separation comparing to the salt gradient, while application of combined pH-salt gradient, resulted in further improvement
Descriptors		CHRYSOSPORIUM ISOENZYMES MANGANESE PEROXIDASES HYDROGEN-ION CONCENTRATION CHROMATOGRAPHY