Author/Editor		Falnoga, Ingrid; Kobal, Alfred B; Stibilj, Vekoslava; Horvat, Milena
Title		Selenoprotein P in subjects exposed to mercury and other stress situations such as physical load or metal chelation treatment
Type		članek
Source		Biol Trace Elem Res
Vol. and No.		Letnik 89
Publication year		2002
Volume		str. 25-33
Language		eng
Abstract		In plasma, Se is found in plasma glutathione peroxidase selenoprotein P, and albumins. The aim of the present study was to determine the influence of lifegoing exposure to various levels of mercury vapor on plasma Se content and the fraction bound to SEl-P. Second, a pilot study was performed on the influence of short-term excessive physical stress and methal chelation treatment. Samples of human plasma/serum obtained from a control group, Idrija residents living in a HG-polluted environment because of the vicinity ofthe Idrija mercury mine, a few Idrija residents exposed to excessive physical stress, and two retired miners treated with the drug Dimaval were investigated. Selenoprotein P was isolated by affinity chromathography, and the concentrations of selenium were determined by radiochemical neutron activation analysis and hydride generation-atomic fluorescence spectrometry.Regardless of the group investigated, plasma Se average values were very similar. A significant change of Sel P was noted only in the group exposed to physical stress as compared to the same subjects before the test, to the control group, and to the Hg exposed group. The decrease of Se bound on Sel-P was accompanied by its increase in fraction of eGSH-P with albumin.
Descriptors		SELENIUM MERCURY CHELATING AGENTS PROTEINS STRESS AGE FACTORS ENVIRONMENTAL EXPOSURE OCCUPATIONAL EXPOSURE NEUTRON ACTIVATION ANALYSIS SPECTROMETRY, FLUORESCENCE UNITHIOL