Author/Editor     Mazej, Darja; Falnoga, Ingrid; Stibilj, Vekoslava
Title     Selenium determination in whole blood, plasma and selenoprotein P by hydride generation atomic fluorescence spectrometry
Type     članek
Source     Acta Chim Slov
Vol. and No.     Letnik 50
Publication year     2003
Volume     str. 185-98
Language     eng
Abstract     Selenium was determined in whole blood, plasma and plasma protein fractions SeP and (GSH-Px+Alb) obtained by affinity chromatography. For the digestion of whole blood and plasma samples a simple digestion procedure using H2SO4, H2O2 and V2O5 in H2SO4 solution was developed, while for the decomposition of plasma protein fractions HN03 and H2O2 were sufficient. After both procedures detection of Se was made by HG-AFS and this combination gave reliable results. Dilution of the GSH-Px+Alb fraction was reduced by lyophilisation of pooled subfractions. The main advantages of the described procedures are low detection limit (0.2 ng Se/g of solution) and the large number of samples analysed in a shori time as only two analyses are needed for determination of the percentage of Se in SeP in plasma sample. The procedures developed were used to determine selenium in real samples from which an average mass fraction of selenium in whole blood of Slovenia residents of 87 +- 13 ng/g (n=43) and Se in SeP of 70 +- 9% (n=5) were found. Both values are comparable with literature data for the general population.
Summary     Za določanje selena v vzorcih krvi in plazme smo razviti postopek, ki povezuje razkroj s H2SO4, H2O in V2O5 v H2SO4 in HG-AFS detekcijski sistem. Za določanje Se v beljakovinskih frakcijah plazme pa je bil dovolj učinkovit razkroj s HN03 in H2O2, ki ravno tako kot prvi v povezavi s HG-AFS daje zanesljive rezultate. Po afinitetni kromatografiji potrebni za izolacijo plazemskih beljakovin, smo subfrakcije frakcije GSH-Px+Alb združili, liofilizirali in tako zmanjšali število določitev iz 16 na eno. Glavni prednosti postopkov sta nizka meja zaznavnosti (0.2 ng Se/g raztopine) in možnost analize velikega števila vzorcev v kratkem časovnem obdobju. Z razvitimi postopki smo določili Se v realnih vzorcih. Povprečen masni delež se v krvi prebivalcev Slovenije je bil 87 +- 13 ng/g (n=43), delež Se vezanega v SeP v plazmi pa je bil 70 +- 9% (n=5). Oba podatka se ujemata s podatki, ki jih navaja literatura za splošno populacijo.
Descriptors     SELENIUM
SELENIUM COMPOUNDS
CHROMATOGRAPHY, AFFINITY
SPECTROMETRY, FLUORESCENCE