Author/Editor		Rupel, Tatjana; Majstorović, Tamara; Šedlbauer, Mitja; Turk, Nataša
Title		Serological identification of Listeria monocytogenes isolated from food using slide agglutination test
Translated title		Serološka klasifikacija sevov Listeria monocytogenes, izoliranih iz živil, z uporabo seroaglutinacijskega testa
Type		članek
Source		Zdrav Vars
Vol. and No.		Letnik 42, št. 4
Publication year		2003
Volume		str. 157-9
Language		eng
Abstract		Listeria monocytogenes is subdivided into 13 serotypes on the basis of somatic (O) and flagellar (H) antigens. Because a vast majority of human disease are caused by only three serotypes (1/2a, 1/2b and 4b), serotyping is of limited value in epidemiologic investigations. Strains of Listeria monocytogenes isolated from food have been collected at the Department of Sanitary Microbiology since 1994. Pure cultures of Listeria recovered from foods were confirmed with the api-Listeria test (bioMerieux). Serological slide agglutination test was done on all Listeria monocytogenes isolates, using commercial antisera Bacto - Listeria O Antisera Types 1,4 and Poly (Difco). The 56 strains obtained were classified into 2 serovars. There were 46 strains of Listeria monocytogenes serotype 1, and six strains of serotype 4; the test for four strains was negative. The aim of subtyping Listeria monocytogenes isolates was to iden.tify serotypes that had been most frequently isolated from food samples.
Summary		Listeria monocytogenes ima 13 serotipov, serološko klasificiranih z aglutinacijskimi testi z antiserumi proti somatskim (O) in flagelarnim (H) antigenom. Večina opisanih okužb s povzročiteljem Listeria monocytogenes se pri Ijudeh zgodi s serotipi 1/2a, 1/2b in 4b. Pri epidemioloških raziskavah ima serotipizacija le omejeno vrednost. V laboratoriju Oddelka za sanitarno mikrobiologijo od leta 1994 zbiramo in hranimo seve povzročitelja Listeria monocytogenes, izolirane iz živil. Pri biokemični potrditvi smo uporabili test api-Listeria (bioMerieux). Seroaglutinacijo sevov smo izvedli s komercialno pripravljenim testom Bacto- Listeria O Antisera Types 1,4 in Poly (Difco). Vseh 56 izoliranih sevov smo serotipizirali v 2 serotipa. Večina, 46 testiranih sevov je serotip 1, šest sevov je serotip 4, ostali štirje sevi pa niso aglutinirali z nobenim antiserumom. Namen našega dela je bil pregledati, kateri serotipi so najpogosteje prisotni pri izolatih povzročitelja Listeria monocytogenes izživil.
Descriptors		FOOD MICROBIOLOGY LISTERIA MONOCYTOGENES SEROTYPING AGGLUTINATION TESTS