Author/Editor		Tozon, N; Štrbenc, M; Fazarinc, G
Title		Določanje limfocitov T v limfomatoznih bezgavkah psov s pomočjo protiteles proti humanemu antigenu CD3
Translated title		Detection of canine T lymphocytes in lymphoma-affected lymph nodes using antibodies directed against the human CD3 antigen
Type		članek
Source		Slov Vet Res
Vol. and No.		Letnik 40, št. 2
Publication year		2003
Volume		str. 77-82
Language		eng
Abstract		The applicability of some commercially available antibodies directed against the human CD3 antigen in detecting canine T lymphocytes in lymphoma-affected lymph nodes was evaluated. Rabbit anti-human CD3 Dako A/S antibodies proved successful in demonstrating canine T cells, even on tissue specimens fixed in formol and embedded in paraffin wax. The antibodies recognize the intracellular fragment of the CD3 molecule, which is relatively resistant to routine histological specimen processing. Other antibodies directed against the CD3 antigen were not successful in determining the T cells, in spite of the application of different antigen-retrieval procedures.
Summary		Ugotavljali smo uporabnost nekaterih komercialno dostopnih protiteles proti humanemu antigenu CD3 za določanje limfocitov T v bezgavkah psov z razvitim limfomom. Kot primerna so se izkazala kunčja protitelesa z oznako Anti-Human CD3 (Dako A/S), ki omogočajo prikaz celic tipa T tudi na tkivnih vzorcih limfomatoznih bezgavk predhodno fiksiranih v formalinu in zalitih v parafin. Protitelesa prepoznajo znotrajcelični fragment CD3 molekule, katerega struktura je razmeroma odporna proti postopkom klasične histološke obdelave vzorcev, kar je za rutinsko patohistološko diagnostiko velikega pomena. Z ostalimi testiranimi protitelesi proti CD3 antigenu nam, kljub uporabi različnih postopkov za odkritje antigena, celic T ni uspelo določiti.
Descriptors		LYMPHOMA, LARGE-CELL, DIFFUSE T-LYMPHOCYTES LYMPH NODES ANTIGENS, CD3 DOGS IMMUNOHISTOCHEMISTRY