Author/Editor		Petelin, M; Pogačnik, A; Pavlica, Z
Title		Primerjava celjenja rane na dlesni po uporabi gingivalne obveze ali brez nje pri psu pasme beagle
Translated title		Comparison of gingival wound healing with and without dressing in the beagle dog
Type		članek
Source		Slov Vet Res
Vol. and No.		Letnik 40, št. 2
Publication year		2003
Volume		str. 101-8
Language		slo
Abstract		A gingival enlargement that is removed by conventional gingivectomy ereates an extensive gingival wound, which heals by secondary intent. In this study the healing effects of different periodontal dressings: soft and non-soluble (Barricaid), and soft and soluble (Reso-Pac) on the gingival wounds of 12 beagle dogs were observed. After preparing the dogs' wound test-sites, the wounds of 4 dogs were covered with Barricaid. Four other animals were treated with Reso-Pac, while the remaining 4 dogs received no post-operative treatment. Biopsies were taken from the wounds for the first seven days after the procedures. The evaluations of the epithelization, inflammation and condition of connective tissue were performed on step-serial sections. In addition, the endothelial cells were immunohistochemically stained with CD31 antibodies to determine vascularity. Four days after the experimental injuries, the epithelization of the wounds treated with Reso-Pac was slightly better in comparison with that of the wounds treated with Barricaid or with the untreated controls. Seven days after the injuries, the wounds of all three experimental groups were covered by non-keratinized epitheliums and rete pegs were developing. The morphometrical analyses of vascularity showed that on the first post-operative day the greatest number of vessels/mm2 was in the wounds treated with Reso-Pac (p<0.01), although this had reduced by the third day as it did in the untreated wounds. The vascularity of Barricaid-treated wounds increased steadily throughout the healing period. The vascularity of the wounds of all three experimental groups increased from the fourth post-operative day onwards and was almost at the same level by the seventh day. From the clinical and histological perspectives, Reso-Pac was found to be the most suitable dressing when compared to Barricaid or to leaving a wound untreated.
Summary		Hiperplastično dlesen odstranimo z gingivotomijo in ob tem povzročimo obširno rano, ki se celi z obilico granulacijskega tkiva. V raziskavi smo primerjali vpliv gingiv'alnih obvez na celjenje rane po uporabi mehke in v ustih nerazgradljive obveze (barricaid) ter mehke in v ustih razgradljive obveze (reso-pack). Uporabili smo 12 psov ženskega spola pasme beagle. Štirim psom smo pokrili ranjeno dlesen z obvezo barricaid, štirim psom z obvezo Reso-pack, štirim pa obveze nismo dali. Po operaciji smo en teden vsak dan jemali vzorce tkiva iz sredine rane. Primerjali smo hitrost razraščanja epitelija iz roba rane, stopnjo vnetja in stanje vezivnega tkiva. Razraščanje žilja smo ugotavljali z barvanjem endotelijskih celic v vzorcih s CD31 protitelesom. Po štirih dneh je bilo nekoliko boljše razraščanje epitelija na rani pod obvezo Reso-pack v primerjavi z rano pokrito z obvezo barricaid, ali rano brez obveze. Po sedmih dneh so bile rane vseh treh skupin pokrite z neporoženevajočim epitelijem z začetki vezivnih jezičkov, ki so se vraščali v epitelij. Prvi dan, ko smo naredili rano, je bilo največ krvnih žil pod obvezo reso-pack (P < 0,01), katerih število se je zmanjševalo do četrtega dne. Podobno se je število žil zmanjševalo tudi pri rani brez obveze, medtem ko se je število krvnih žil pod obvezo barricaid med celjenjem povečevalo. Sedmi dan poskusa je bilo število krvnih žil enako pri vseh ranah. Iz kliničnega in histološkega pregleda lahko sklenemo, da je obveza reso-pack najustreznejša, če jo primerjamo z gingivalno obvezo barricaid ali mestom, kjer obveze nismo namestili.
Descriptors		GINGIVECTOMY WOUND HEALING PERIODONTAL DRESSINGS DOGS