Author/Editor | Krt, B | |
Title | Vpliv uporabe antigenov faze I in faze II Coxiella burnetii na serološko diagnostiko mrzlice Q pri govedu | |
Translated title | The influence of Coxiella burnetii phase I and phase II antigens on the serological diagnosis of Q fever in cattle | |
Type | članek | |
Source | Slov Vet Res | |
Vol. and No. | Letnik 40, št. 3-4 | |
Publication year | 2003 | |
Volume | str. 207-11 | |
Language | slo | |
Abstract | The aim of our work was to study the influence of C. burnetii phase I and phase II antigens when used in the serological diagnosis of Q fever in cattle. Samples were obtained from a cattle herd with an outbreak of Q fever and examined, using the phase I and phase II antigens (together and separately), in ELISA and complement fixation tests (CFT). The CFT indicated that 11 sera (39%) were positive when both antigens were used, 3 (11%) were positive using only the phase I antigen and 2 sera (7%) were positive to phase II. The ELISA examinations revealed that 16 sera (57%) were positive when both antigens were used and 3 (11%) were positive using only the phase I antigen. No positive sera were found using the phase II antigen only. In milk testing there were 9 (32%) ELISA-positive samples using both phases and 5 (18%) were positive when the phase I antigen was used separately. The phase II antigen only demonstrated one positive milk sample (4%). It was concluded that both antigens should be used simultaneously in CFT, whereas the phase I antigen could be sufficient for herd-level ELISA tests. | |
Summary | Namen našega dela je bil ugotoviti vpliv uporabe antigenov faze I in faze II C. burnetii na serološko diagnostiko mrzlice Q pri govedu. V raziskavi smo uporabili vzorce iz črede, kjer je bila ugotovljena mrzlica Q. Preiskovali smo jih z metodama reakcije vezanja komplementa (RVK) in ELISA, v katerih smo uporabili antigene faze I in faze II (skupaj in ločeno). V RVK je pri uporabi antigenov obeh faz reagiralo pozitivno 11 (39%) vzorcev, 3 (11%) vzorci so bili pozitivni samo na fazo I, 2 (7%) pa le na fazo II. V testu ELISA je bilo pri uporabi antigenov obeh faz pozitivnih 16 (57%) in pri uporabi faze I 3 (11%) serumi, medtem ko nobeden ni bil pozitiven samo na fazo II. Pri mlečnih vzorcih smo v testu ELISA pri uporabi antigenov obeh faz ugotovili 9 (32%) pozitivnih vzorcev, pri uporabi antigenov faze I 5 (18%) in pri fazi II 1 (4%). Ugotovili smo, da je potrebno pri RVK uporabljati antigene obeh faz hkrati, medtem ko bi za testiranje črede v testu ELISA lahko zadoščala uporaba antigenov faze I. | |
Descriptors | Q FEVER COXIELLA BURNETII CATTLE ENZYME-LINKED IMMUNOSORBENT ASSAY COMPLEMENT FIXATION TESTS |