Author/Editor		Jeraj, Nataša; Romih, Rok; Lenasi, Helena; Breskvar, Katja
Title		In situ detection of progesterone binding sites in the plasma membrane of the filamentous fungus Rhizopus nigricans
Type		članek
Source		Acta Chim Slov
Vol. and No.		Letnik 50
Publication year		2003
Volume		str. 757-62
Language		eng
Abstract		Steroid hydroxylating enzymes in the fungus Rhizopus nigricans are induced by progesterone and by some other steroids. It is known that in higher organisms steroids exert their nongenomic action via steroid binding proteins located in the plasma membrane of the cells, thus our aim was to detect progesterone binding sites in R. nigricans plasma membrane. In this report membrane receptors were identified by two independent methods, analysis of progesterone binding to plasma membrane fraction by competitive binding assay using [3H]- and [1H] progesterone (ECSO=55 nM) and in situ binding of fluorescein isothiocyanate-coupled cell impermeant progesterone conjugate to fungal protoplasts, detected by fluorescence microscopy.
Descriptors		PROGESTERONE CELL MEMBRANE PROTEIN BINDING BINDING SITES BINDING, COMPETITIVE RHIZOPUS FLUORESCEIN-5-ISOTHIOCYANATE MICROSCOPY, FLUORESCENCE PROTOPLASTS RECEPTORS, PROGESTERONE RADIOLIGAND ASSAY