Author/Editor     Košnik, I; Wraber, B; Košnik, M; Ihan, A
Title     Sulphidoleukotriene release from peripheral blood mononuclear cells in chronic urticaria and anaphylaxis
Type     članek
Source     Allergy Hypersens Asth
Vol. and No.     Letnik 1, št. 2
Publication year     2003
Volume     str. 20-5
Language     eng
Abstract     Sulphidoleukotrienes (sLT) are important mediators of allergic and pseudoallergic diseases. We studied the ability of leukocytes of patients with allergic and pseudoallergic diseases to release sLT. Leukocytes of 18 patients with Hymenoptera venom allergy (10 atopics, 8 non-atopics), 14 patients with chronic urticaria (CU) and I I healthy volunteers were in-vitro stimulated with antibodies against high affinity IgE receptors (antiFceRI) and ionomycin and the amount of released sLT was measured by EL1SA assay. Spontaneous and anti-FceRI induced release of sLT was diminished in patients with CU. Ionomycine induced release of sLT in CU patients was similar to that of healthy controls but it was significantly higher in patients with allergic diseases. The anti-FceRI induced release of sLT from leukocytes of the atopic allergic patients was significantly higher than that of sLT from leukocytes of the healthy controls and the non-atopic allergic patients. We speculate that weaker response to anti-FceRI and normal response to ionomycine in leukocytes of CU patients might be due to the lower number of available FceRI on basophils (lower number or occupancy with blocking autoantibodies). Higher sLT releasability of leukocytes of allergic patients after stimulation with ionomycine might be a mechanism, responsoble for the development of overt allergic disease in patients with specific IgE antibodies.
Descriptors     WASP VENOMS
ANAPHYLAXIS
URTICARIA
LEUKOTRIENES
BEE VENOMS
LEUKOCYTES, MONONUCLEAR
IONOMYCIN
RECEPTORS, IGE
DOSE-RESPONSE RELATIONSHIP, DRUG
ENZYME-LINKED IMMUNOSORBENT ASSAY