Author/Editor		Kostanjšek, Rok; Avguštin, Gorazd; Drobne, Damjana; Štrus, Jasna
Title		Morphological and molecular examination of bacteria associated with the wall of the papillate region of the gut in Porcellio scaber (Isopoda)
Type		članek
Source		In: Sfenthourakis S, de Araujo PB, Hornung E, et al, editors. Proceedings of the 5th international symposium on the biology of terrestrial isopods; 2001 May. Leiden: Koninklijke Brill NV,
Publication year		2004
Volume		str. 103-20
Language		eng
Abstract		Terrestrial isopods play an important role in Icaf litter decomposition and, thereforc, in the decomposition of complex organic molecules. Sinee the presence of autochthonous microbiota in thc isopod gut has not been directly proven yet, thc microbial community associated with the papillatc region of the terrestrial isopod Porcellio scaber was examined more thoroughly. Electron and light microscopy were used to describe the morphology of rod and tilamentous baeteria associated with the cuticular lining ol' the papillate region. These observations revealed a specific attachment mechanism, which enables the bactcria to remain in the hindgut. Molecular biological approaches were used to determine the genetic diversity and the phylogenetic affiliations of bactcria residing in thc papillate region, where high concentrations of bacteria are commonly found. In spite of the thorough rinsing of the isolated gut, a diverse microbiota was found to be associated with thc hindgut. "fhc phylogenelic analysis showed that most of the bacteria arc closely related to bactcria commonly inhabiting animal gastrointcstinal tracts, whereas some represent new, so far undcscribcd taxa. As expected, bactcria related to typical soil bacteria, such as the genera Bacillus and Pseudomonas, wera also found. Thc presence of bacteria closely related to anacrobcs from thc intestine of higher animals, and bacteria specifically attached to the gut wall in the papillatc region indicate, that in addition to ubiquitous microorganisms that are passed through the isopod gut with food, there might also exist microorganisms that are adapted to live in the hindgut. These represent, therefore, the true autochthonous microorganisms in the gut of P. seaber.
Descriptors		INTESTINES BACTERIA PHYLOGENY CRUSTACEA MICROSCOPY, ELECTRON BASE SEQUENCE DNA POLYMERASE CHAIN REACTION RNA, RIBOSOMAL, 16S