Author/Editor		Kladnik, Aleš; Vilhar, Barbara; Chourey, Prem S; Dermastia, Marina
Title		Sucrose synthase isozyme SUS1 in the maize root cap is preferentially localized in the endopolyploid outer cells
Type		članek
Source		Can J Bot
Vol. and No.		Letnik 82, št. 1
Publication year		2004
Volume		str. 93-103
Language		eng
Abstract		The structure of the maize (Zen mny.s L.) root cap was studied to quantitttively evaluate the relationship among the size of the cells, their endopolyploidy level, and the abundance of the sucrose synthase isozyme SUSI. Median longitudinal root cap sections were analysed using immunoiocalization, quantitative DNA staining, and image cytometry. Both the immunolocalization signal for the SUSI prutein and the endopolyploidy level increased from caLyptrogen towards the root cap periphery and were thus the highest in the outer cells. These cells had a nuclear DNA content of mostly SC or higher and the lzirgest volumes of all root cap cells. The high amount of SUS 1 protein in the outer, endopolyploid cells suggests an association between endoreduplication and the abundance of this enzyme. The outer cells are involved in mucilage production: hence, there is a possibility that sucrose synthase provides monosaccharide precursors for mucilage synthesis.
Descriptors		CORN PLANT ROOT CAP SUCROSE ISOENZYMES DNA CELL SIZE IMAGE CYTOMETRY