Author/Editor		Zorn, Branko
Title		Pomen granulocitne elastaze, kisikovih radikalov in enoverižne DNK spermijev za diagnostiko in prognozo prikritega vnetja spolovil pri neplodnih moških
Translated title		Relevance of seminal granulocyte elastase, reactive oxygen species and single-stranded sperm DNA for the diagnosis and prognosis of silent genital tract inflammation in infertile men
Type		monografija
Place		Ljubljana
Publisher		Medicinska fakulteta
Publication year		2003
Volume		str. 95
Language		eng
Abstract		Introduction: By combining the assessment of the complex elastase-inhibitor in semen (s-EI), a marker of silent male genital tract inflammation (MGTI), spermiogram, reactive oxygen species (ROS) and single-stranded sperm DNA as indicators of sperm function, this work is an original approach to precise the incidence of silent MGTI in infertile males, to explore the effect of s-EI on sperm characteristics and function, and to evaluate the influence of s-EI levels on the results of classical in vitro fertilization (IVF). Methods: In 416 male partners of infertile couples, we obtained the spermiogram, and assessed s-EI by immunoassay, ROS by chemiluminescence and DNA denaturation by acridine orange test. We defined the incidence of inflammation according to the level of s-EI. We looked for changes in clinical and sperm characteristics, and ROS and DNA denaturation modifications according to s-EI levels. Results of IVF and ICSI were analyzed according to ROS and s-EI levels, respectively. Statistical analyses were performed using ROC curve, correlation tests and multiple linear regression. Results: a)`s-EI as indicator of silent MGTI. s-EI at the cut-off level of >= 290 micro g/I was found to be efficient (sensitivity: 79.5% specificity: 74.4%) in the detection of silent MGTI as defined by leukocytospermia (> 1x106 leukocytes/ml of ejaculate). Increased (>= 290 micro g/I) and high (> 1000 micro g/I) s-EI levels were observed in 34% and 16% of male partners of infertile couples, respectively. In non-leukocytospermic men, increased and high s-EI levels were found in 25% and 11%, respectively. Fertile men were characterised by a low incidence (5%) of increased s-EI. b) s-EI and clinical and semen characteristics, and DNA denaturation and ROS. Increased levels of s-EI were associated with an older male's age and partner's damaged tubes. (Abstract truncated at 2000 characters).
Descriptors		LEUKOCYTE ELASTASE REACTIVE OXYGEN SPECIES DNA, SINGLE-STRANDED INFERTILITY, MALE SEMEN GENITAL DISEASES, MALE INFLAMMATION FERTILIZATION IN VITRO SPERM COUNT SPERM MOTILITY LEUKOCYTE COUNT CIPROFLOXACIN