Author/Editor		Čadež, Neža; Štorman, Alenka
Title		Metodologija mikrobiološke analitike s poudarkom na novejših mikrobioloških metodah
Translated title		Methodology of microbiological analyses with emphasis on novel microbiological methods
Type		članek
Source		In: Gašperlin L, Žlender B, editors. Varnost živil. 22. Bitenčevi živilski dnevi 2004; 2004 mar 18-19; Radenci. Ljubljana: Biotehniška fakulteta, Oddelek za živilstvo,
Publication year		2004
Volume		str. 81-92
Language		slo
Abstract		Microbiological analysis for detection of food-borne spoilage microorganisms and food pathogens is a standard practice for ensuring food safety and quality. Novel methods for the detection microorganisms in food are an alternative to traditional detection and identification methods. These novel methods have several advantages such as speed, low detection limit and the ability of automation. But for their standardization several criteria such as diagnostic accuracy, detection limit, high speed, costs, and simplicity must be fulfilled. The present review focuses on novel microbiological methods for the detection of foodborne microorganisms (PCR detection, hybridization methods and real-time PCR), their advantages and deficiencies. For discrimination of food-borne pathogens on different levels the genotyping methods are useful for epidemiological and taxonomical studies.
Descriptors		FOOD MICROBIOLOGY SENSITIVITY AND SPECIFICITY POLYMERASE CHAIN REACTION NUCLEIC ACID HYBRIDIZATION NUCLEIC ACID PROBES