Author/Editor		Vilfan, Tanja; Črešnar, Bronislava; Fournier, Didier; Stojan, Jure; Breskvar, Katja
Title		Characterisation and expression of a gene encoding a mutarotase from the fungus Rhizopus nigricans
Type		članek
Source		FEMS Microbiol Lett
Vol. and No.		Letnik 235
Publication year		2004
Volume		str. 101-8
Language		eng
Abstract		A gene coding for a mutarotase was isolated and characterised from the filamentous fungus Rhizopus nigricans. In order to determine the encoded enzyme's activity a recombinant protein was prepared in the baculovirus expression system and the mutarotase activity was determined. Expression studies showed that the gene is repressed by high as well as low concentrations of glucose and derepressed during deficiency of glucose. Besides the regulation at the level of transcription, an accelerative effect of glucose in growth medium on the mutarotase mRNA decay was also demonstrated. Moreover, a Southern hybridisation performed at lower temperatures suggested that the R. nigricans genome harbours a nucleotide sequence, that is homologous to the isolated gene.
Descriptors		RHIZOPUS GALACTOSE URIDINE DIPHOSPHATE GLUCOSE EPIMERASE GENE EXPRESSION REGULATION, FUNGAL RECOMBINANT FUSION PROTEINS GLUCOSE TRANSCRIPTION, GENETIC GENOME, FUNGAL GENOMIC LIBRARY HYBRIDIZATION BASE SEQUENCE POLYMERASE CHAIN REACTION DNA, COMPLEMENTARY CLONING, MOLECULAR