| Author/Editor | Šarc, Lucija | |
| Title | Mehanizem letalnih učinkov poly APS, toksina izoliranega iz morske spužve Reniera sarai | |
| Type | monografija | |
| Place | Ljubljana | |
| Publisher | Medicinska fakulteta | |
| Publication year | 2003 | |
| Volume | str. 108 | |
| Language | slo | |
| Abstract | Poly- APS, toxic water soluble 3-alkylpyridinium salts, were isolated from the marine sponge Reniera sarai. Till now, different authors reported that poly- APS in vitro strongly inhibits acetyl cholinesterase (AChE), has hemolytic activity, forms pores in cellular membranes, interacts with serum proteins, binds to lipids and aggregates small unilamellar lipid vesicles. In vivo experiments showed that the AChE-inhibitory effects, presented as transient bradycardia and an increase of the residual respiratory volume, could be observed only with lower doses of the toxin. At higher doses the AChE-inhibitory effects were not observed as they were probably masked by other faster and more pronounced lethal effects of the toxin, such as the initiation of blood coagulation, platelet aggregation or other toxic effects of the toxin. It is not known whether the plugs seen on the histological samples are primarily formed due to the direct interaction of poly-APS with plasma components or whether they are the result of the toxins' action on the platelet and vascular walls. The aim of present study is to evaluate the role of platelet aggregation as the mechanism of the toxin's lethality in vivo. At first, we evaluated effects of the toxin on the platelet in vitro by optical aggregometry. The mechanism of platelet aggregation induced by poly APS was studied with aggregation inhibitory substances with known mechanism of action: clopidogrel, acetylsalicylic acid and eptifibatide. The importance of in vitro effects of poly APS on platelet aggregation as well as the importance of antiplatelet substances in the toxin lethality was tested in vivo. Three groups of Wistar rats were treated with poly APS. The first group was treated with lethal doses (3,5 mg/kg) of poly APS, the second group with sublethal doses (1,4 mg/kg) of poly APS. (Abstract truncated at 2000 characters) | |
| Descriptors | PORIFERA TOXINS PYRIDINIUM COMPOUNDS PLATELET AGGREGATION PLATELET AGGREGATION INHIBITORS ASPIRIN AUTOPSY LUNG KIDNEY ENDOTHELIUM, VASCULAR RATS, WISTAR |