Author/Editor		Boh, Bojana; Berovič, Marin; Wraber, Branka; Hodžar, Damjan; Habijanič, Jožica; Pohleven, Franc; Zore, Irena
Title		Ganoderma lucidum (W.Curt.:Fr.) Lloyd and G. applanatum (Pers.) Pat. (Aphyllophoromycetideae) from Slovenian habitats: cultivation, isolation, and testing of active compounds
Type		članek
Source		International journal of medicinal mushrooms
Vol. and No.		Letnik 6, št. 1
Publication year		2004
Volume		str. 15-32
Language		eng
Abstract		Original strains of Ganoderma lucidum (W Curt.:Fr ) Lloyd and G. applanatum (Pers.) Pat. (synonymous G. lipsiense (Batsch) Atk.) from Slovenian habitats were isolated and used for experimental work In G. applanaium, research was focused on triterpenoid acids.They were isolated irom the fruiting bodies of G. applanatum of Istrian origin (BFWS Gaa2), growing on Populus trees.The highest amount of triterpenoid acids was found in the tubes (6.4 mg/g of air-dried weight), followed by the younger dark context layer of the pileus (2.5 mg/g), the older white context layer (0.6 mg/g), and the upper surface of the fruiting body (0.6 mg/g). G. lucidum strain MZHI G97, isolated from the Slovenian forest, was cultivated by solid state cultivation in a horizontal reactor and by submerged liquid substrate cultivation in a 10-L laboratory stirred tank reactor. Using a 17% (wet weight) of 6-day-old vegetative inoculum, up to 17.0 gL-1 of dry fungai biomass was obtained in a fed batch process. From the mycelium biomass, 1.7 gL-1 of extracellular and 0.45 gL-1 of intracellular polysaccharides were isolated, which were mainly beta-D-glucans. Polysaccharide fractions, tested in vitro on the induction of cytokine svnthesis in primary cultures of human mononuclear cells from a buffy coat of healthy donors, induced 3.0-630 pg mL-1 of TNF-alpha and 1.23-2.18 pg mL-1 of IFN-gamma.
Descriptors		BASIDIOMYCETES TRITERPENES POLYSACCHARIDES MONOCYTES BIOMASS TREES TUMOR NECROSIS FACTOR INTERFERON TYPE II ENZYME-LINKED IMMUNOSORBENT ASSAY CHROMATOGRAPHY, ION EXCHANGE NUCLEAR MAGNETIC RESONANCE